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	Provedor de dados Electron. J. Biotechnol. (3) Autor Al-Salamah,Ali A (3) El-Tayeb,Mohamed A (3) Elbadawi,Yahya B (3) El-Toni,Ahmed Mohamed (2) Ibrahim,Abdelnasser S.S. (2) Ibrahim,Abdelnasser S.S (1) Mais... Palavra-chave Cyclodextrin glucanotransferase (3) Immobilization (2) Amphibacillus sp (1) Amphibacillus sp. NPST-10 (1) Bacillus agaradhaerens (1) Cyclodextrinc (1) Cyclodextrins (1) Double mesoporous core-shell silica nanospheres (1) Enzymatic reaction (1) Magnetic nanoparticle (1) Silica (1) Starch (1) Mais... Tipo do documento Journal article (3) Ano 2014 (1) 2013 (1) 2011 (1) País Chile (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core-shell silica nanospheres Electron. J. Biotechnol. Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B. Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process of the nanospheres included preparing core magnetic magnetite (Fe3O4) nanoparticles, coating the Fe3O4 with a dense silica layer, followed by further coating with functionalized or non-functionalized mesoporous silica shell. The structure of the synthesized nanospheres was characterized using TEM, XRD, and FT-IR analyses. CGTase was immobilized onto the functionalized and non-functionalized nanospheres by covalent attachment and physical adsorption. Results... Tipo: Journal article Palavras-chave: Amphibacillus sp; Cyclodextrin glucanotransferase; Cyclodextrins; Double mesoporous core-shell silica nanospheres; Immobilization. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200001 Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11 Electron. J. Biotechnol. Ibrahim,Abdelnasser S.S; El-Tayeb,Mohamed A; Elbadawi,Yahya B; Al-Salamah,Ali A. The effects of reaction conditions on cyclodextrins (CDs) production by CGTase from newly isolated Bacillus agaradhaerens KSU-A11 is reported. Among six types of starch tested, potato starch gave highest starch conversion into CDs. In addition, CDs yield was about three fold higher when using gelatinized potato starch in comparison to raw starch. The total CDs production was increased with increasing pH, showing maximum starch conversion at pH 10. Furthermore, the proportion of γ-CD was relatively higher under slightly acidic-neutral conditions than at alkaline pH with a maximum proportion of 35.6% at pH 7 compared to 7.6% at pH 10. Maximum starch conversion into CDs was seen at reaction temperature of 55ºC. Lower reaction temperature led to... Tipo: Journal article Palavras-chave: Bacillus agaradhaerens; Cyclodextrin glucanotransferase; Cyclodextrinc; Enzymatic reaction; Starch. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500004 Immobilization of cyclodextrin glucanotransferase on aminopropyl-functionalized silica-coated superparamagnetic nanoparticles Electron. J. Biotechnol. Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B. Background: Cyclodextrin glycosyltransferase (CGTase) from Amphibacillus sp. NPST-10 was successfully covalently immobilized on aminopropyl-functionalized silica coated superparamagnetic nanoparticles; and the properties of immobilized enzyme were investigated. The synthesis process included preparing of core magnetic magnetite (Fe3O4) nanoparticles using solvothermal synthesis; followed by coating of Fe3O4 nanoparticles with dense amino-functionalized silica (NH2-SiO2) layer using in situ functionalization method. The structure of synthesized Fe3O4@NH2-SiO2 nanoparticles was characterized using TEM, XRD, and FT-IR analysis. Fe3O4@NH2-SiO2 nanoparticles were further activated by gluteraaldehyde as bifunctional cross linker, and the activated nanoparticles... Tipo: Journal article Palavras-chave: Amphibacillus sp. NPST-10; Cyclodextrin glucanotransferase; Immobilization; Magnetic nanoparticle; Silica. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600010 Registros recuperados: 3 Primeira ... 1 ... Última

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